MPP | 四川农大许强团队揭示TaRBP1稳定TaGLTP并负调控对小麦条锈病抗性
2023/6/26 15:20:06 阅读：33 发布者：
以下文章来源于植物胁迫与抗逆研究 ，作者吴昕扬
近日，由四川农大许强团队在MPP发表了题为《TaRBP1 stabilizes TaGLTP and negatively regulates stripe rust resistance in wheat》的研究论文，揭示了TaRBP1稳定TaGLTP并负调控对小麦条锈病抗性。
鞘脂代谢产物的动态平衡和分布调节程序性细胞死亡和植物防御的水平。然而，关于鞘脂代谢和植物防御之间关系的分子机制，目前的知识仍然有限。
在本研究中，作者鉴定了小麦RNA结合蛋白1（TaRBP1）和及其mRNA积累在小麦条锈病菌Pst感染后显著减少。通过病毒诱导的基因沉默敲除TaRBP1，增强了宿主植物活性氧（ROS）的积累和细胞死亡，赋予了对Pst的强烈抗性，表明TaRBP1可能是对Pst响应的负调节因子。
TaRBP1在植物中形成均聚物并与TaRBP1 C末端相互作用。此外，TaRBP1与鞘氨醇转移蛋白TaGLTP发生物理相互作用。敲除TaGLTP增强了小麦对Pst的抗性。
鞘氨醇脂质代谢产物分别在TaGLTP沉默的小麦和TaRBP1沉默的小麦中表现出显著的积累。在TaRBP1蛋白存在的情况下，TaGLTP未能在植物中以26S蛋白酶体依赖的方式降解。
作者的研究结果揭示了一种新的易感机制，通过该机制，植物通过稳定TaGLTP的积累来微调其防御反应，从而在Pst感染期间抑制ROS和鞘脂的积累。
The dynamic balance and distribution of sphingolipid metabolites modulate the level of programmed cell death and plant defence. However, current knowledge is still limited regarding the molecular mechanism underlying the relationship between sphingolipid metabolism and plant defence. In this study, we identified a wheat RNA-binding protein 1 (TaRBP1) and TaRBP1 mRNA accumulation significantly decreased in wheat after infection by Puccinia striiformis f. sp. tritici (Pst). Knockdown of TaRBP1 via virus-induced gene silencing conferred strong resistance to Pst by enhancing host plant reactive oxygen species (ROS) accumulation and cell death, indicating that TaRBP1 may act as a negative regulator in response to Pst. TaRBP1 formed a homopolymer and interacted with TaRBP1 C-terminus in plants. Additionally, TaRBP1 physically interacted with TaGLTP, a sphingosine transfer protein. Knockdown of TaGLTP enhanced wheat resistance to the virulent Pst CYR31. Sphingolipid metabolites showed a significant accumulation in TaGLTP-silenced wheat and TaRBP1-silenced wheat, respectively. In the presence of the TaRBP1 protein, TaGLTP failed to be degraded in a 26S proteasome-dependent manner in plants. Our results reveal a novel susceptible mechanism by which a plant fine-tunes its defence responses by stabilizing TaGLTP accumulation to suppress ROS and sphingolipid accumulation during Pst infection.
本文转载自植物胁迫与抗逆研究
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