PCE | 过氧化氢调节水稻中的Osa-miR156-OsSPL2/OsTIFY11b模块
2023/5/29 16:31:54 阅读：56 发布者：
以下文章来源于植物胁迫与抗逆研究 ，作者吴昕扬
近日，台湾大学郑石通团队在PCE发表了题为《Hydrogen peroxide regulates the Osa-miR156-OsSPL2/OsTIFY11b module in rice》的研究论文，揭示了过氧化氢调节水稻中的Osa-miR156-OsSPL2/OsTIFY11b模块的作用。
田间种植的水稻在暴露于各种环境胁迫时，会产生大量的活性氧，如H2O2。小RNA（miRNA）在植物应激反应中起着至关重要的作用。本研究对水稻中H2O2调节的miRNA的功能进行了表征。
小RNA深度测序显示，H2O2处理后miR156水平下降。对水稻转录组和降解组数据库的搜索表明，OsSPL2和OsTIFY11b是miR156靶基因。miR156与OsSPL2和OsTIFY11b之间的相互作用通过农杆菌的瞬时表达测定得到证实。此外，在过表达miR156的转基因水稻植物中，OsSPL2和OsTIFY11b转录本的水平低于野生型植物。
OsSPL2-GFP和OsTIFY11b-GFP蛋白定位于细胞核。酵母双杂交和双分子荧光互补分析表明OsSPL2和OsTIFY11b之间存在相互作用。此外，OsTIFY11b与OsMYC2相互作用以调节编码蛋白酶抑制剂的OsRBBI3-3的表达。
本研究的结果表明，H2O2在水稻中的积累抑制了miR156的表达，并诱导其靶基因OsSPL2和OsTIFY11b的表达，这两个基因的蛋白质在细胞核中相互作用以调节参与植物防御反应的OsRBBI3-3的表达。
Field-grown rice (Oryza sativa L.), when exposed to various environmental stresses, produces high amounts of reactive oxygen species, such as H2O2. MicroRNAs (miRNAs) play crucial roles in plant stress responses. This study characterized the functions of H2O2-regulated miRNAs in rice. Small RNA deep sequencing revealed that miR156 levels decreased following H2O2 treatment. Searches of the rice transcriptome and degradome databases indicated that OsSPL2 and OsTIFY11b are miR156-target genes. Interactions between miR156 and OsSPL2 and OsTIFY11b were confirmed using transient expression assays through agroinfiltration. In addition, the levels of OsSPL2 and OsTIFY11b transcripts were lower in transgenic rice plants overexpressing miR156 than in wild-type plants. The OsSPL2-GFP and OsTIFY11b-GFP proteins were localized to the nucleus. Yeast two-hybrid and bimolecular fluorescence complementation assays indicated interactions between OsSPL2 and OsTIFY11b. Furthermore, OsTIFY11b interacted with OsMYC2 to regulate the expression of OsRBBI3-3, which encodes a proteinase inhibitor. The results suggested that H2O2 accumulation in rice suppresses the expression of miR156, and induces the expression of its target genes, OsSPL2 and OsTIFY11b, whose proteins interact in the nucleus to regulate the expression of OsRBBI3-3, which is involved in plant defense.
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