MPP：西北农林大学园艺学院王西平团队揭示转录因子VaERF16和VaMYB306相互作用增强葡萄对灰霉病菌的抗性

今天介绍的是西北农林大学园艺学院王西平团队在MPP发表的研究论文《The transcription factors VaERF16 and VaMYB306 interact to enhance resistance of grapevine to Botrytis cinerea infection》。

灰霉菌Botrytis cinerea是一种感染栽培葡萄的真菌；鉴定和表征宿主的抗性机制对葡萄产业非常重要。 

在本研究中，作者报告了来自中国野葡萄的乙烯反应因子 (ERF) 家族成员 VaERF16在 B. cinerea 感染期间以及对激素乙烯和茉莉酸甲酯处理后表达。

VaERF16在拟南芥中的异源过表达通过水杨酸和茉莉酸/乙烯信号通路显著增强了对灰霉病菌和丁香假单胞菌DC3000 的抗性。酵母双杂交、双分子荧光互补和免疫共沉淀测定表明VaERF16与家族转录因子VaMYB306 相互作用。

葡萄叶中VaERF16 或 VaMYB306的过表达增加了对B. cinerea 的抗性，并导致编码防御素样蛋白的防御相关基因PDF1.2的上调。相反，任一基因的沉默导致对B. cinerea的易感性增加。酵母单杂交和双荧光素酶测定表明，VaERF16通过直接结合其启动子中的GCC框来增加VaPDF1.2的转录水平。

值得注意的是，单独的VaMYB306不与VaPDF1.2启动子结合，但 VaERF16-VaMYB306转录复合物导致VaPDF1.2的转录水平更高，这表明蛋白质通过它们的相互作用发挥作用。

本研究阐明的该调节模块可能对增强葡萄对B. cinerea 感染的抗性具有一定价值。

Botrytis cinerea is a fungus that infects cultivated grape (Vitis vinifera); the identification and characterization of resistance mechanisms in the host is of great importance for the grape industry. Here, we report that a transcription factor in the ethylene-responsive factor (ERF) family (VaERF16) from Chinese wild grape (Vitis amurensis ‘Shuang You’) is expressed during B. cinerea infection and in response to treatments with the hormones ethylene and methyl jasmonate. Heterologous overexpression of VaERF16 in Arabidopsis thaliana substantially enhanced resistance to B. cinerea and the bacterium Pseudomonas syringae DC3000 via the salicylic acid and jasmonate/ethylene signalling pathways. Yeast two-hybrid, bimolecular fluorescence complementation, and co-immunoprecipitation assays indicated that VaERF16 interacts with the MYB family transcription factor VaMYB306. Overexpression of VaERF16 or VaMYB306 in grape leaves increased resistance to B. cinerea and caused an up-regulation of the defence-related gene PDF1.2, which encodes a defensin-like protein. Conversely, silencing of either gene resulted in increased susceptibility to B. cinerea. Yeast one-hybrid and dual-luciferase assays indicated that VaERF16 increased the transcript levels of VaPDF1.2 by binding directly to the GCC box in its promoter. Notably, VaMYB306 alone did not bind to the VaPDF1.2 promoter, but the VaERF16–VaMYB306 transcriptional complex resulted in higher transcript levels of VaPDF1.2, suggesting that the proteins function through their mutual interaction. Elucidation of this regulatory module may be of value in enhancing resistance of grapevine to B. cinerea infection.

转自：植物科学SCI

如有侵权，请联系本站删除！