Plant Cell：扬州大学高勇团队揭示光敏色素互作因子通过协调红光和脱落酸调节气孔开度

今天介绍的是由扬州大学高勇团队在Plant Cell上发表的研究论文《Phytochrome Interacting Factor regulates stomatal aperture by coordinating red light and abscisic acid》。

气孔是协调光合作用固定二氧化碳和控制叶片蒸腾失水的重要阀门。光敏色素互作因子(PIF)是红光反应的负调控因子，属于碱性螺旋-环-螺旋(BHLH)转录因子家族。本文中，作者证明了水稻PIF家族基因OsPIL15是气孔开度的负调节基因并控制水稻的蒸腾作用。OsPIL15通过激活水稻脱落酸不敏感蛋白5(OsABI5)来减少气孔开度，而OsABI5是水稻脱落酸（ABA）信号传导的重要正向调节因子。此外，OsPIL15也会与NIGT1/HRS1/HHO家族转录因子水稻HRS1同源物3(OsHH03)相互作用，可以增强对气孔开度的调节。值得注意的是，作者发现与OsPIL15同源的玉米(Zea Mays)PIF家族基因ZmPIF1和ZmPIF3也参与了玉米气孔开度的调节，这表明PIF介导的气孔开度调节在植物谱系中可能是保守的。作者的发现解释了PIFs在红光介导的气孔开放中发挥作用的分子机制，并表明PIFs可以通过协调红光和ABA信号通路来调节气孔开度。

Stomata are crucial valves coordinating the fixation of carbon dioxide by photosynthesis and water loss through leaf transpiration. Phytochrome interacting factors (PIFs) are negative regulators of red light responses that belong to the basic helix-loop-helix (bHLH) family of transcription factors. Here, we show that the rice (Oryza sativa) PIF family gene OsPIL15 acts as a negative regulator of stomatal aperture to control transpiration in rice. OsPIL15 reduces stomatal aperture by activating rice ABSCISIC ACID INSENSITIVE 5 (OsABI5), which encodes a critical positive regulator of abscisic acid (ABA) signaling in rice. Moreover, OsPIL15 interacts with the NIGT1/HRS1/HHO family transcription factor rice HRS1 HOMOLOG 3 (OsHHO3) to possibly enhance the regulation of stomatal aperture. Notably, we discovered that the maize (Zea mays) PIF family genes ZmPIF1 and ZmPIF3, which are homologous to OsPIL15, are also involved in the regulation of stomatal aperture in maize, indicating that PIF-mediated regulation of stomatal aperture may be conserved in the plant lineage. Our findings explain the molecular mechanism by which PIFs play a role in red-light-mediated stomatal opening, and demonstrate that PIFs regulate stomatal aperture by coordinating the red light and ABA signaling pathways.

原文链接: https://doi.org/10.1093/plcell/koac244

转自：植物科学SCI

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