PCE: 组成型表达基因JAR1能诱导植物产生分子变化从而更好地抵御干旱

今天介绍的是由德国科学家Ute C. Vothknecht在PCE上发表的《Constitutive expression of JASMONATE RESISTANT 1 induces molecular changes that prime the plants to better withstand drought》。

本研究中，作者研究了能将茉莉酸(JA)转化为茉莉酸-L-异亮氨酸(JA-Ile) 的JAR1酶水平改变的拟南芥植株。对一个新构建的JAR1过表达系(35S::JAR1)的分析表明，在35S::JAR1中结构性增加的JA-Ile产量会影响了植物的生长发育，导致生长不良和开花延迟。且在干旱胁迫条件下，35S::JAR1与野生型相比会表现出更好的抗旱性。相比之下，JA-Ile含量下降的JAR1-11植株却对干旱高度敏感。对正常和干旱条件下植物的RNA-seq分析和植物激素分析为研究JA-Ile含量变化引起的分子重编程提供了深入了解。

此外35S::JAR1植株表现出与生长和开花相关的发育基因表达的变化以及与干旱的适应系统有关的进一步的转录差异变化，其中包括气孔密度和开度，以及活性氧(ROS)的产生和消除。对野生型和携带roGFP-Orp1传感器的Jar1-11植物的分析证明了JA-Ile能在减缓MeV诱导的过氧化氢的过程中产生作用。作者的数据证实了JA-Ile在非生物胁迫反应中的作用，并表明由JAR1介导的JA-Ile含量的增加能提升拟南芥对干旱胁迫耐性。

In this work we investigated Arabidopsis thaliana plants with altered levels of the enzyme JASMONATE RESISTANT 1 (JAR1) that converts jasmonic acid (JA) to jasmonyl-L-isoleucine (JA-Ile). Analysis of a newly generated over-expression line (35S::JAR1) revealed that constitutively increased JA-Ile production in 35S::JAR1 alters plant development, resulting in stunted growth and delayed flowering. Under drought-stress conditions, 35S::JAR1 plants showed reduced wilting and recovered better from desiccation than the wild type. By contrast, jar1-11 plants with a strong reduction in JA-Ile content were hypersensitive to drought. RNA-seq analysis and hormonal profiling of plants under normal and drought conditions provided insights into the molecular reprogramming caused by the alteration in JA-Ile content. Especially 35S::JAR1 plants displayed changes in expression of developmental genes related to growth and flowering. Further transcriptional differences pertained to drought-related adaptive systems, including stomatal density and aperture but also reactive oxygen species (ROS) production and detoxification. Analysis of wild type and jar1-11 plants carrying the roGFP-Orp1 sensor support a role of JA-Ile in the alleviation of MeV-induced H2 O2 production. Our data substantiate a role of JA-Ile in abiotic stress response and suggest that JAR1-mediated increase in JA-Ile content primes Arabidopsis towards improved drought stress tolerance. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.

原文链接：https://doi.org/10.1111/pce.14402

转自：植物科学SCI

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