JXB：北林大付玉杰-孟冬团队揭示褪黑素通过促进类黄酮合成增强木豆对盐胁迫的耐受性

今天介绍的是北林大付玉杰-孟冬团队在JXB发表的研究论文《Melatonin enhances plant stress tolerance by promoting flavonoid enrichment, focusing on luteolin for salt stress》。

褪黑素通过参与植物代谢物的生物合成来提高对多种胁迫的耐受性。黄酮类化合物作为重要的植物次生代谢产物，被广泛认为与抗性有关。然而，褪黑素和类黄酮之间的互作仍然是未知的。 

在本研究中，作者通过褪黑激素预处理，发现木豆对盐、干旱和热胁迫的抗性显著增强。结合转录组和 LC-ESI-MS/MS 代谢组学表明，褪黑激素显着诱导类黄酮富集并介导生物合成途径基因的重编程。  

CcF3'H家族编码一种催化木犀草素生物合成的酶，响应褪黑激素处理下，上调表达倍数最为显著。转录因子CcPCL1直接与CcF3'H-5启动子结合以增强 CcF3'H-5的转录。 

此外，盐胁迫还诱导了CcPCL1和CcF3'H-5的表达。通过过表达CcPCL1 和 CcF3'H-5，能够促进木犀草素的生物合成，极大地增强了耐盐性。 

总体而言，本研究的结果表明，褪黑激素预处理通过CcPCL1和CcF3'H-5途径促进了木犀草素的生物合成，导致木豆耐盐性增强。褪黑激素通过介导类黄酮生物合成增强植物对多种胁迫的耐受性，为研究褪黑激素与类黄酮之间的交叉互作提供了新思路。

Melatonin improves plant resistance to multiple stresses by participating biosynthesis of plant metabolites. Flavonoids, as an important family of plant secondary metabolites, are widely recognized to be involved in resistance. However, the crosstalk between melatonin and flavonoid is largely unknown. With melatonin pretreatment, we discovered the resistance of pigeon pea was significantly enhanced against salt, drought, and heat stresses. Combined transcriptome and LC-ESI-MS/MS metabolomics showed that melatonin significantly induced the enrichment of flavonoids and mediated the reprogramming of biosynthetic pathway genes. The CcF3'H family encodes an enzyme that catalyzes the biosynthesis of luteolin, with the highest upregulated expression fold in response to melatonin treatment. The transcription factor CcPCL1 directly bonded to the CcF3'H-5 promoter to enhance the transcription of CcF3'H-5. In addition, salt stress also induced the expression of CcPCL1 and CcF3'H-5. Further, through the overexpression of CcPCL1 and CcF3'H-5, salt tolerance was greatly enhanced by promoting the biosynthesis of luteolin. Overall, our results indicated that melatonin pretreatment promoted luteolin biosynthesis through the CcPCL1 and CcF3'H-5 pathways, resulting in salt tolerance in pigeon pea. Our study showed that melatonin enhanced plant tolerance to multiple stresses by mediating flavonoid biosynthesis, providing new ideas for studying the crosstalk between melatonin and flavonoids.

转自：植物生物技术Pbj

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