2022.11.07~2022.11.13
CNS刊登文章
01
Science
2022/11/10
“Structures of a mobile intron retroelement poised to attack its structured DNA target”
II型内含子是核酶，催化它们的自我剪切，并作为入侵DNA的逆转录元件发挥作用。作为逆转录转座子，II型内含子形成核糖核蛋白(RNP)复合体，在基因组中漫游，通过整合反向的前剪接。来自美国耶鲁大学的Anna Marie Pyle课题组发现逆转录转位是通过结构精细的核酶、其蛋白质迁移因子和结构化DNA底物形成的三元复合体来实现的。研究者解析了一个包含有完整的IIC组内含子成熟酶逆转录元件的正准备整合到DNA茎环基序中的冷冻电镜结构，通过将在靶向DNA之前和之后的RNP分别进行可视化，研究者发现它为攻击做好了准备，并且与它的目标DNA完美匹配。本研究揭示了一个典型的逆转录元件的设计原则，并更证实了II类内含子是遗传多样性的古老元件的假设。
原文链接
https://www.science.org/doi/10.1126/science.abq2844
02
Cell
2022/11/10
“Molecular recognition of morphine and fentanyl by the human μ-opioid receptor”
吗啡和芬太尼是最常用的阿片类药物之一，阿片类药物通过作用于G蛋白偶联受体家族中的阿片受体，尤其是μ型阿片受体（μ opioid receptor, μOR），主要激活下游抑制性Gi/o蛋白从而发挥镇痛等药理活性。
来自中国科学院上海药物研究所徐华强团队、谢欣团队和王明伟团队合作解析了与吗啡和芬太尼结合的人类μ型阿片受体（μOR）-G蛋白复合物的结构，并揭示了它们如何与受体结合的关键差异。研究人员还报告了μOR与TRV130、PZM21和SR17018结合的结构，并揭示了这些激动剂与配体结合袋的TM3侧而不是TM6/7侧的优先相互作用。相反，吗啡和芬太尼与TM3和TM6/7区域形成双重相互作用。TM6/7界面的突变废除了吗啡和芬太尼促进的阻遏蛋白对μOR的招募。旨在减少TM6/7相互作用的配体显示出优先的G蛋白信号传导。这些研究结果为芬太尼对μOR的识别和信号传导提供了关键的见解，这可能有助于合理设计下一代镇痛药。
原文链接
https://www.cell.com/cell/fulltext/S0092-8674(22)01260-0
Nature
本周无
2022.11.07~2022.11.13
子刊刊登文章
01
Cell Research
11.10
“A variant-proof SARS-CoV-2 vaccine targeting HR1 domain in S2 subunit of spike protein”
02
Molecular Cell
11.07
“ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation”
03
Nature Structural & Molecular Biology
11.07
1.“Cryo-EM structures of prefusion SIV envelope trimer”
11.07
2.“A structural biology community assessment of AlphaFold2 applications”
11.09
3.“Anionic lipids unlock the gates of select ion channels in the pacemaker family”
04
Nature Communications
11.07
1.“Structure of the IscB–ωRNA ribonucleoprotein complex, the likely ancestor of CRISPR-Cas9”
11.07
2.“Functionalized graphene grids with various charges for single-particle cryo-EM”
11.07
3.“An active site loop toggles between conformations to control antibiotic hydrolysis and inhibition potency for CTX-M β-lactamase drug-resistance enzymes”
11.07
4.“Structural basis of organic cation transporter-3 inhibition”
11.08
5.“Structural basis for sequence-independent substrate selection by eukaryotic wobble base tRNA deaminase ADAT2/3”
11.08
6.“Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1”
11.09
7.“CryoEM structures of the multimeric secreted NS1, a major factor for dengue hemorrhagic fever”
11.09
8.“Design of synthetic collagens that assemble into supramolecular banded fibers as a functional biomaterial testbed”
11.09
9.“Structural dynamics of AAA + ATPase Drg1 and mechanism of benzo-diazaborine inhibition”
11.09
10.“Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold
11.09
11.“Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE”
11.10
12. “Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation”
11.10
13.“The 3D structure of lipidic fibrils of α-synuclein”
11.11
14.“Protein control of photochemistry and transient intermediates in phytochromes”
11.11
15.“Design and directed evolution of noncanonical β-stereoselective metalloglycosidases”
11.11
16.“Structural basis of human SNAPc recognizing proximal sequence element of snRNA promoter”
11.11
17.“Dominant ARF3 variants disrupt Golgi integrity and cause a neurodevelopmental disorder recapitulated in zebrafish”
11.12
18.“TLR3 forms a highly organized cluster when bound to a poly(I:C) RNA ligand”
11.12
19.“Lipid-mediated prestin organization in outer hair cell membranes and its implications in sound amplification”
11.12
20.“Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel”
05
Science Advances
11.09
1.“Cryo-EM structures of peripherin-2 and ROM1 suggest multiple roles in photoreceptor membrane morphogenesis”
11.09
2.“Cryo-EM structure of the human sodium-chloride cotransporter NCC”
转自：“北京生物结构前沿研究中心”微信公众号
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